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Home>Product>Primary Antibody

Phospholipase C Gamma 1 Recombinant Rabbit mAb

#1212

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Recombinant Antibody
Western blotting analysis using phospholipase c gamma 1 antibody (Cat#1212). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospholipase C gamma 1 antibody (Cat#1212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse spleen using phospholipase c gamma 1 antibody (Cat#1212, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Phospholipase C gamma 1 expression in HT-1080 cells using Phospholipase C gamma 1 antibody (Cat#1212, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospholipase C gamma 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PLCG1
  • Catalog：
    1212
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospholipase C Gamma 1 Recombinant Rabbit mAb
Aliases PLCG1; Phospholipase C Gamma 1; PLC-II 2; PLC148; NCKAP3; PLC1; 1-Phosphatidylinositol 4,5-Bisphosphate Phosphodiesterase Gamma-1; Phospholipase C, Gamma 1 (Formerly Subtype 148); Phosphoinositide Phospholipase C-Gamma-1; Phospholipase C-II ; PLC-Gamma-1; EC 3.1.4.11; PLCgamma1; PLC-148; 1-Phosphatidyl-D-Myo-Inositol-4,5-Bisphosphate; Monophosphatidylinositol Phosphodiesterase; Triphosphoinositide Phosphodiesterase; Phosphatidylinositol Phospholipase ; Phosphoinositide Phospholipase C; Inositoltrisphosphohydrolase; Phospholipase C, Gamma 1; Phospholipase C-Gamma-1; Phosphoinositidase C; Phospholipase C-148; PLCGAMMA1
Background

Gene Name: PLCG1

NCBI Gene Entry: 5335

UniProt Entry: P19174

Application Information

Molecular Weight: Predicted, 149 kDa; observed, 149 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2235

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human PLC gamma 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using phospholipase c gamma 1 antibody (Cat#1212). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospholipase C gamma 1 antibody (Cat#1212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded mouse spleen using phospholipase c gamma 1 antibody (Cat#1212, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of Phospholipase C gamma 1 expression in HT-1080 cells using Phospholipase C gamma 1 antibody (Cat#1212, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospholipase C gamma 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PLCG1
  • Catalog：
    1212
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospholipase c gamma 1 antibody (Cat#1212). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospholipase C gamma 1 antibody (Cat#1212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse spleen using phospholipase c gamma 1 antibody (Cat#1212, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Phospholipase C gamma 1 expression in HT-1080 cells using Phospholipase C gamma 1 antibody (Cat#1212, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospholipase C gamma 1.
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