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Home>Product>Primary Antibody

Crystallin Alpha B Recombinant Rabbit mAb

#1397

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Recombinant Antibody
Western blotting analysis using Crystallin alpha B antibody (Cat#1397). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Crystallin alpha B antibody (Cat#1397, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Crystallin alpha B antibody (Cat#1397, 1:1,000). Nuclei were stained blue with DAPI; Crystallin alpha B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Crystallin alpha B expression in H9c2 cells using Crystallin alpha B antibody (Cat#1397, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Crystallin alpha B.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CRYAB
  • Catalog：
    1397
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    R

Information

Data

Information
Product Name Crystallin Alpha B Recombinant Rabbit mAb
Aliases CRYAB; Crystallin Alpha B; HSPB5; CRYA2; Renal Carcinoma Antigen NY-REN-27; Heat Shock Protein Beta-5; Rosenthal Fiber Component; Alpha-Crystallin B Chain; Epididymis Secretory Protein Li 101; Heat-Shock 20 KD Like-Protein; Crystallin, Alpha B; Alpha(B)-Crystallin; HEL-S-101; CTRCT16; CMD1II; CTPP2; HspB5; MFM2
Background

Gene Name: CRYAB

NCBI Gene Entry: 1410

UniProt Entry: P02511

Application Information

Molecular Weight: Predicted, 20 kDa; observed, 19 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3500

Species Reactivity: Rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human CRYAB
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Crystallin alpha B antibody (Cat#1397). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Crystallin alpha B antibody (Cat#1397, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with Crystallin alpha B antibody (Cat#1397, 1:1,000). Nuclei were stained blue with DAPI; Crystallin alpha B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Crystallin alpha B expression in H9c2 cells using Crystallin alpha B antibody (Cat#1397, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Crystallin alpha B.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CRYAB
  • Catalog：
    1397
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    R
RELATED PRODUCTS
Western blotting analysis using Crystallin alpha B antibody (Cat#1397). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Crystallin alpha B antibody (Cat#1397, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with Crystallin alpha B antibody (Cat#1397, 1:1,000). Nuclei were stained blue with DAPI; Crystallin alpha B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Crystallin alpha B expression in H9c2 cells using Crystallin alpha B antibody (Cat#1397, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Crystallin alpha B.
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