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CD40L Recombinant Rabbit mAb

#1594

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Recombinant Antibody
Western blotting analysis using CD40L antibody (Cat#1594). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD40L antibody (Cat#1594, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD40L expression in H9c2 cells using CD40L antibody (Cat#1594, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD40L.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CD40LG
  • Catalog:
    1594
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name CD40L Recombinant Rabbit mAb
Aliases CD40LG; CD40 Ligand; CD40-L; CD40L; TRAP; TNF-Related Activation Protein; HCD40L; TNFSF5; CD154; HIGM1; T-BAM; Tumor Necrosis Factor (Ligand) Superfamily Member 5; T-B Cell-Activating Molecule; CD40 Antigen Ligand; T-Cell Antigen Gp39; Gp39; IMD3; Tumor Necrosis Factor (Ligand) Superfamily, Member 5 (Hyper-IgM Syndrome); Tumor Necrosis Factor Ligand Superfamily Member 5; Hyper-IgM Syndrome; CD154 Antigen; GP39; IGM
Background

Gene Name: CD40LG

NCBI Gene Entry: 959

UniProt Entry: P29965

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3380

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human CD40L
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD40L antibody (Cat#1594). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD40L antibody (Cat#1594, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of CD40L expression in H9c2 cells using CD40L antibody (Cat#1594, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD40L.
Western blotting analysis using CD40L antibody (Cat#1594). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD40L antibody (Cat#1594, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD40L expression in H9c2 cells using CD40L antibody (Cat#1594, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD40L.
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