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Home>Product>Primary Antibody

ADAMTS13 Recombinant Rabbit mAb

#1650

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Recombinant Antibody
Western blotting analysis using ADAMTS13 antibody (Cat#1650). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ADAMTS13 antibody (Cat#1650, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with ADAMTS13 antibody (Cat#1650, 1:1,000). Nuclei were stained blue with DAPI; ADAMTS13 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ADAMTS13 expression in HeLa cells using ADAMTS13 antibody (Cat#1650, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ADAMTS13.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ADAMTS13
  • Catalog：
    1650
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ADAMTS13 Recombinant Rabbit mAb
Aliases ADAMTS13; ADAM Metallopeptidase With Thrombospondin Type 1 Motif 13; VWF-CP; VWFCP; A Disintegrin-Like And Metalloprotease (Reprolysin Type) With Thrombospondin Type 1 Motif, 13; A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13; Von Willebrand Factor-Cleaving Protease; VWF-Cleaving Protease; MGC118899; MGC118900; ADAM-TS13; ADAMTS-13; FLJ42993; C9orf8; TTP; DKFZp434C2322; DKFZP434C2322; EC 3.4.24.87; EC 3.4.24.14; EC 3.4.24.82; ADAM-TS 13; C9ORF8
Background

Gene Name: ADAMTS13

NCBI Gene Entry: 11093

UniProt Entry: Q76LX8

Application Information

Molecular Weight: Predicted, 154 kDa; observed, 154 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3925

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ADAMTS13
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ADAMTS13 antibody (Cat#1650). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ADAMTS13 antibody (Cat#1650, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with ADAMTS13 antibody (Cat#1650, 1:1,000). Nuclei were stained blue with DAPI; ADAMTS13 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ADAMTS13 expression in HeLa cells using ADAMTS13 antibody (Cat#1650, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ADAMTS13.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ADAMTS13
  • Catalog：
    1650
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ADAMTS13 antibody (Cat#1650). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ADAMTS13 antibody (Cat#1650, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with ADAMTS13 antibody (Cat#1650, 1:1,000). Nuclei were stained blue with DAPI; ADAMTS13 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ADAMTS13 expression in HeLa cells using ADAMTS13 antibody (Cat#1650, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ADAMTS13.
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