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Home>Product>Primary Antibody

Hsp90 α Recombinant Rabbit mAb

#1668

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Recombinant Antibody
Western blotting analysis using Hsp90 α antibody (Cat#1668). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp90 α antibody (Cat#1668, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Hsp90 α antibody (Cat#1668, 1:1,000). Nuclei were stained blue with DAPI; Hsp90 α was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Hsp90 α expression in HepG2 cells using Hsp90 α antibody (Cat#1668, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Hsp90 α.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSP90AA1
  • Catalog：
    1668
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Hsp90 α Recombinant Rabbit mAb
Aliases HSP90AA1; Heat Shock Protein 90 Alpha Family Class A Member 1; HSP90N; HSPC1; HSPCA; Heat Shock Protein 90kDa Alpha (Cytosolic), Class A Member 1; Lipopolysaccharide-Associated Protein; Heat Shock 90kDa Protein 1, Alpha; Renal Carcinoma Antigen NY-REN-38; Heat Shock 90kD Protein 1, Alpha; Heat Shock Protein HSP 90-Alpha; LPS-Associated Protein; Heat Shock 86 KDa; FLJ31884; HSP90A; HSP 86; Hsp89; Hsp90; HSP86; LAP-2; Heat Shock Protein 90kDa Alpha Family Class A Member 1; Epididymis Secretory Sperm Binding Protein Li 65p; Epididymis Luminal Secretory Protein 52; Heat Shock 90kD Protein 1, Alpha-Like 4; Heat Shock 90kD Protein, Alpha-Like 4; EC 3.6.4.10; HEL-S-65p; HSPCAL1; HSPCAL4; HSP89A; Hsp103; HSP89; HSP90; EL52; HSPN; LAP2
Background

Gene Name: HSP90AA1

NCBI Gene Entry: 3320

UniProt Entry: P07900

Application Information

Molecular Weight: Predicted, 85 kDa; observed, 85 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3995

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Hsp90 alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Hsp90 α antibody (Cat#1668). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp90 α antibody (Cat#1668, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Hsp90 α antibody (Cat#1668, 1:1,000). Nuclei were stained blue with DAPI; Hsp90 α was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Hsp90 α expression in HepG2 cells using Hsp90 α antibody (Cat#1668, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Hsp90 α.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HSP90AA1
  • Catalog：
    1668
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Hsp90 α antibody (Cat#1668). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsp90 α antibody (Cat#1668, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Hsp90 α antibody (Cat#1668, 1:1,000). Nuclei were stained blue with DAPI; Hsp90 α was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Hsp90 α expression in HepG2 cells using Hsp90 α antibody (Cat#1668, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Hsp90 α.
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