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Home>Product>Primary Antibody

PSB9 / LMP2 Recombinant Rabbit mAb

#1694

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Recombinant Antibody
Western blotting analysis using PSB9 / LMP2 antibody (Cat#1694). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSB9 / LMP2 antibody (Cat#1694, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with PSB9 / LMP2 antibody (Cat#1694, 1:1,000). Nuclei were stained blue with DAPI; PSB9 / LMP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PSB9 / LMP2 expression in HT-1080 cells using PSB9 / LMP2 antibody (Cat#1694, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSB9 / LMP2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PSMB9
  • Catalog：
    1694
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PSB9 / LMP2 Recombinant Rabbit mAb
Aliases PSMB9; Proteasome 20S Subunit Beta 9; RING12; PSMB6i; LMP2; Multicatalytic Endopeptidase Complex Chain 7; Really Interesting New Gene 12 Protein; Large Multifunctional Peptidase 2; Proteasome Subunit Beta Type-9; Low Molecular Mass Protein; Proteasome Subunit Beta-1i; Proteasome Subunit Beta 9; Proteasome Chain 7; Macropain Chain 7; EC 3.4.25.1; Beta1i; Proteasome (Prosome, Macropain) Subunit, Beta Type, 9 (Large Multifunctional Peptidase 2); Proteasome (Prosome, Macropain) Subunit, Beta Type, 9 (Large Multifunctional Protease 2); Proteasome (Prosome, Macropain) Subunit, Beta Type, 9; Proteasome Catalytic Subunit 1i; Proteasome Subunit Beta 6i; Proteasome Subunit Beta1i; Proteasome-Related Gene; Proteasome Subunit Β1i; PRAAS3; BETA1I; PSMB6I
Background

Gene Name: PSMB9

NCBI Gene Entry: 5698

UniProt Entry: P28065

Application Information

Molecular Weight: Predicted, 23 kDa; observed, 23 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4110

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Proteasome 20S LMP2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PSB9 / LMP2 antibody (Cat#1694). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSB9 / LMP2 antibody (Cat#1694, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with PSB9 / LMP2 antibody (Cat#1694, 1:1,000). Nuclei were stained blue with DAPI; PSB9 / LMP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PSB9 / LMP2 expression in HT-1080 cells using PSB9 / LMP2 antibody (Cat#1694, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSB9 / LMP2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PSMB9
  • Catalog：
    1694
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PSB9 / LMP2 antibody (Cat#1694). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSB9 / LMP2 antibody (Cat#1694, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with PSB9 / LMP2 antibody (Cat#1694, 1:1,000). Nuclei were stained blue with DAPI; PSB9 / LMP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PSB9 / LMP2 expression in HT-1080 cells using PSB9 / LMP2 antibody (Cat#1694, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PSB9 / LMP2.
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