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DCR2 Recombinant Rabbit mAb

#1834

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Recombinant Antibody
Western blotting analysis using DCR2 antibody (Cat#1834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCR2 antibody (Cat#1834, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with DCR2 antibody (Cat#1834, 1:1,000). Nuclei were stained blue with DAPI; DCR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DCR2 expression in C2C12 cells using DCR2 antibody (Cat#1834, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DCR2.
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Specification:
Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    TNFRSF10D
  • Catalog:
    1834
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    M, R
Information
Product Name DCR2 Recombinant Rabbit mAb
Aliases TNFRSF10D; TNF Receptor Superfamily Member 10d; TRAILR4; TRUNDD; CD264; DCR2; Tumor Necrosis Factor Receptor Superfamily, Member 10d, Decoy With Truncated Death Domain; Tumor Necrosis Factor Receptor Superfamily Member 10D; TNF-Related Apoptosis-Inducing Ligand Receptor 4; TRAIL Receptor With A Truncated Death Domain; TRAIL Receptor 4; Decoy Receptor; TRAIL-R4; DcR2; TNF Receptor-Related Receptor For TRAIL; CD264 Antigen
Background

Gene Name: TNFRSF10D

NCBI Gene Entry: 8793

UniProt Entry: Q9UBN6

Application Information

Molecular Weight: Predicted, 42 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4685

Species Reactivity: Mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human DCR2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using DCR2 antibody (Cat#1834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCR2 antibody (Cat#1834, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with DCR2 antibody (Cat#1834, 1:1,000). Nuclei were stained blue with DAPI; DCR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of DCR2 expression in C2C12 cells using DCR2 antibody (Cat#1834, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DCR2.
Western blotting analysis using DCR2 antibody (Cat#1834). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCR2 antibody (Cat#1834, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with DCR2 antibody (Cat#1834, 1:1,000). Nuclei were stained blue with DAPI; DCR2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DCR2 expression in C2C12 cells using DCR2 antibody (Cat#1834, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, DCR2.
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