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MPP1 Recombinant Rabbit mAb

#1860

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Recombinant Antibody
Western blotting analysis using MPP1 antibody (Cat#1860). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MPP1 antibody (Cat#1860, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with MPP1 antibody (Cat#1860, 1:1,000). Nuclei were stained blue with DAPI; MPP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    MPP1
  • Catalog:
    1860
  • Application:
    WB, ICC
  • Species Reactivity:
    H, M
Information
Product Name MPP1 Recombinant Rabbit mAb
Aliases MPP1; MAGUK P55 Scaffold Protein 1; DXS552E; PEMP; Membrane Protein, Palmitoylated 1, 55kDa; 55 KDa Erythrocyte Membrane Protein; Membrane Protein, Palmitoylated 1; Membrane Palmitoylated Protein 1; EMP55; P55; Palmitoylated Erythrocyte Membrane Protein; Membrane Protein, Palmitoylated 1 (55kD); Erythrocyte Membrane Protein P55; Aging-Associated Gene 12; Migration-Related Gene; EC 2.7.11; AAG12; MRG1
Background

Gene Name: MPP1

NCBI Gene Entry: 4354

UniProt Entry: Q00013

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4770

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human MPP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using MPP1 antibody (Cat#1860). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MPP1 antibody (Cat#1860, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with MPP1 antibody (Cat#1860, 1:1,000). Nuclei were stained blue with DAPI; MPP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using MPP1 antibody (Cat#1860). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MPP1 antibody (Cat#1860, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with MPP1 antibody (Cat#1860, 1:1,000). Nuclei were stained blue with DAPI; MPP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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