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Home>Product>Primary Antibody

14-3-3 Recombinant Rabbit mAb

#1881

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Recombinant Antibody
Western blotting analysis using 14-3-3 antibody (Cat#1881). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with 14-3-3 antibody (Cat#1881, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with 14-3-3 Tubulin antibody (Cat#1881, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of 14-3-3 expression in HepG2 cells using 14-3-3 antibody (Cat#1881, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, 14-3-3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    YWHAB/YWHAZ
  • Catalog：
    1881
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name 14-3-3 Recombinant Rabbit mAb
Aliases YWHAB; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Alpha Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Beta Polypeptide; 14-3-3 Protein Beta/Alpha; 14-3-3 Alpha; Protein 1054; KCIP-1; YWHAA; Protein Kinase C Inhibitor Protein-1; Protein Kinase C Inhibitor Protein 1; Epididymis Secretory Protein Li 1; 14-3-3 Beta; HEL-S-1; GW128; HS1; YWHAZ; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta; KCIP-1; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Delta Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Zeta Polypeptide; 14-3-3 Protein Zeta/Delta; 14-3-3 Delta; 14-3-3-Zeta; 14-3-3 Zeta; YWHAD; Tyrosine 3/Tryptophan 5 -Monooxygenase Activation Protein, Zeta Polypeptide; 14-3-3 Protein/Cytosolic Phospholipase A2; Protein Kinase C Inhibitor Protein-1; Protein Kinase C Inhibitor Protein 1; Epididymis Secretory Protein Li 93; Epididymis Secretory Protein Li 3; Epididymis Luminal Protein 4; Phospholipase A2; 14-3-3-ZETA; HEL-S-93; HEL-S-3; POPCHAS; HEL4
Background

Gene Name: YWHAB/YWHAZ

NCBI Gene Entry: 7529 , 7534

UniProt Entry: P31946 , P63104

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4830

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human 14-3-3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using 14-3-3 antibody (Cat#1881). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with 14-3-3 antibody (Cat#1881, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with 14-3-3 Tubulin antibody (Cat#1881, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of 14-3-3 expression in HepG2 cells using 14-3-3 antibody (Cat#1881, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, 14-3-3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    YWHAB/YWHAZ
  • Catalog：
    1881
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using 14-3-3 antibody (Cat#1881). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with 14-3-3 antibody (Cat#1881, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with 14-3-3 Tubulin antibody (Cat#1881, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of 14-3-3 expression in HepG2 cells using 14-3-3 antibody (Cat#1881, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, 14-3-3.
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