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Home>Product>Primary Antibody

CYBB Recombinant Rabbit mAb

#1921

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Recombinant Antibody
Western blotting analysis using CYBB antibody (Cat#1921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYBB antibody (Cat#1921, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with CYBB antibody (Cat#1921, 1:1,000). Nuclei were stained blue with DAPI; CYBB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CYBB expression in HT-1080 cells using CYBB antibody (Cat#1921, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CYBB.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYBB
  • Catalog：
    1921
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name CYBB Recombinant Rabbit mAb
Aliases CYBB; Cytochrome B-245 Beta Chain; NOX2; Cytochrome B-245 Heavy Chain; Cytochrome B558 Subunit Beta; NADPH Oxidase 2; GP91-PHOX; Superoxide-Generating NADPH Oxidase Heavy Chain Subunit; Heme-Binding Membrane Glycoprotein Gp91phox; Neutrophil Cytochrome B 91 KDa Polypeptide; Cytochrome B(558) Subunit Beta; P22 Phagocyte B-Cytochrome; CGD91-Phox; GP91PHOX; P91-PHOX; CGD; Cytochrome B-245, Beta Polypeptide; Cytochrome B-245 Beta Polypeptide; Chronic Granulomatous Disease; Gp91-Phox; EC 1.6.3; AMCBX2; GP91-1; Gp91-1; IMD34; CGDX
Background

Gene Name: CYBB

NCBI Gene Entry: 1536

UniProt Entry: P04839

Application Information

Molecular Weight: Predicted, 65 kDa; observed, 65 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5135

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human NOX2/CYBB/gp91phox
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CYBB antibody (Cat#1921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYBB antibody (Cat#1921, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with CYBB antibody (Cat#1921, 1:1,000). Nuclei were stained blue with DAPI; CYBB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CYBB expression in HT-1080 cells using CYBB antibody (Cat#1921, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CYBB.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CYBB
  • Catalog：
    1921
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CYBB antibody (Cat#1921). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CYBB antibody (Cat#1921, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with CYBB antibody (Cat#1921, 1:1,000). Nuclei were stained blue with DAPI; CYBB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CYBB expression in HT-1080 cells using CYBB antibody (Cat#1921, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CYBB.
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