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CDC42EP1 Recombinant Rabbit mAb

#2049

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Recombinant Antibody
Western blotting analysis using CDC42EP1 antibody (Cat#2049). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC42EP1 antibody (Cat#2049, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with CDC42EP1 antibody (Cat#2049, 1:1,000). Nuclei were stained blue with DAPI; CDC42EP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CDC42EP1 expression in HeLa cells using CDC42EP1 antibody (Cat#2049, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CDC42EP1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CDC42EP1
  • Catalog:
    2049
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name CDC42EP1 Recombinant Rabbit mAb
Aliases CDC42EP1; CDC42 Effector Protein 1; MSE55; CEP1; Binder Of Rho GTPases 5; BORG5; 55 KDa Bone Marrow Stromal/Endothelial Cell Protein; CDC42 Effector Protein (Rho GTPase Binding) 1; Serum Constituent Protein; Cdc42 Effector Protein 1; Serum Protein MSE55; Borg5
Background

Gene Name: CDC42EP1

NCBI Gene Entry: 11135

UniProt Entry: Q00587

Application Information

Molecular Weight: Predicted, 40 kDa; observed, 60 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5745

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human CDC42EP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CDC42EP1 antibody (Cat#2049). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC42EP1 antibody (Cat#2049, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with CDC42EP1 antibody (Cat#2049, 1:1,000). Nuclei were stained blue with DAPI; CDC42EP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CDC42EP1 expression in HeLa cells using CDC42EP1 antibody (Cat#2049, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CDC42EP1.
Western blotting analysis using CDC42EP1 antibody (Cat#2049). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDC42EP1 antibody (Cat#2049, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with CDC42EP1 antibody (Cat#2049, 1:1,000). Nuclei were stained blue with DAPI; CDC42EP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CDC42EP1 expression in HeLa cells using CDC42EP1 antibody (Cat#2049, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CDC42EP1.
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