Western blotting analysis using HAPLN1 antibody (Cat#2209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HAPLN1 antibody (Cat#2209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with HAPLN1 antibody (Cat#2209, 1:1,000). Nuclei were stained blue with DAPI; HAPLN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HAPLN1 expression in C2C12 cells using HAPLN1 antibody (Cat#2209, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HAPLN1.
HAPLN1; Hyaluronan And Proteoglycan Link Protein 1; CRTL1; Cartilage-Linking Protein 1; Proteoglycan Link Protein; Cartilage Link Protein; Cartilage-Link Protein; Cartilage Linking Protein 1; CRT1
Western blotting analysis using HAPLN1 antibody (Cat#2209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HAPLN1 antibody (Cat#2209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with HAPLN1 antibody (Cat#2209, 1:1,000). Nuclei were stained blue with DAPI; HAPLN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HAPLN1 expression in C2C12 cells using HAPLN1 antibody (Cat#2209, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HAPLN1.
Western blotting analysis using HAPLN1 antibody (Cat#2209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HAPLN1 antibody (Cat#2209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with HAPLN1 antibody (Cat#2209, 1:1,000). Nuclei were stained blue with DAPI; HAPLN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HAPLN1 expression in C2C12 cells using HAPLN1 antibody (Cat#2209, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HAPLN1.
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