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Nucleoporin 50 Recombinant Rabbit mAb

#2285

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Recombinant Antibody
Western blotting analysis using Nucleoporin 50 antibody (Cat#2285). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nucleoporin 50 antibody (Cat#2285, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Nucleoporin 50 antibody (Cat#2285, 1:1,000). Nuclei were stained blue with DAPI; Nucleoporin 50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Nucleoporin 50 expression in HepG2 cells using Nucleoporin 50 antibody (Cat#2285, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Nucleoporin 50.
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Specification:
Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    NUP50
  • Catalog:
    2285
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name Nucleoporin 50 Recombinant Rabbit mAb
Aliases Nucleoporin 50; NPAP60L; Nuclear Pore Complex Protein Nup50; 50 KDa Nucleoporin; Nucleoporin 50kDa; Nucleoporin Nup50; Nuclear Pore-Associated Protein 60 KDa-Like; Nuclear Pore-Associated Protein 60L; Nucleoporin 50kD; NPAP60
Background

Gene Name: NUP50

NCBI Gene Entry: 10762

UniProt Entry: Q9UKX7

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB135

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human NUP50
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Nucleoporin 50 antibody (Cat#2285). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nucleoporin 50 antibody (Cat#2285, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Nucleoporin 50 antibody (Cat#2285, 1:1,000). Nuclei were stained blue with DAPI; Nucleoporin 50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Nucleoporin 50 expression in HepG2 cells using Nucleoporin 50 antibody (Cat#2285, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Nucleoporin 50.
Western blotting analysis using Nucleoporin 50 antibody (Cat#2285). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nucleoporin 50 antibody (Cat#2285, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Nucleoporin 50 antibody (Cat#2285, 1:1,000). Nuclei were stained blue with DAPI; Nucleoporin 50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Nucleoporin 50 expression in HepG2 cells using Nucleoporin 50 antibody (Cat#2285, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Nucleoporin 50.
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