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Home>Product>Primary Antibody

NRAS/HRAS/KRAS Recombinant Rabbit mAb

#2303

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Recombinant Antibody
Western blotting analysis using KRAS+HRAS+NRAS antibody (Cat#2303). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRAS+HRAS+NRAS antibody (Cat#2303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with KRAS+HRAS+NRAS antibody (Cat#2303, 1:1,000). Nuclei were stained blue with DAPI; KRAS+HRAS+NRAS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of KRAS+HRAS+NRAS expression in HeLa cells using KRAS+HRAS+NRAS antibody (Cat#2303, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, KRAS+HRAS+NRAS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NRAS/HRAS/KRAS
  • Catalog：
    2303
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name NRAS/HRAS/KRAS Recombinant Rabbit mAb
Aliases NRAS Proto-Oncogene, GTPase; N-Ras; Neuroblastoma RAS Viral (V-Ras) Oncogene Homolog; Neuroblastoma RAS Viral Oncogene Homolog; Transforming Protein N-Ras; GTPase NRas; V-Ras Neuroblastoma RAS Viral Oncogene Homolog; Proto-Oncogene GTPase; N-Ras Protein Part 4; EC 3.6.5.2; ALPS4; NRAS1; HRAS1; CMNS; KRAS; NCMS; NS6; HRas Proto-Oncogene, GTPase; HRAS1; V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog; Harvey Rat Sarcoma Viral Oncogene Homolog; Transforming Protein P21; GTPase HRas; P21ras; Ras Family Small GTP Binding Protein H-Ras; Harvey Rat Sarcoma Viral Oncoprotein; Transformation Gene: Oncogene HAMSV; GTP- And GDP-Binding Peptide B; Ha-Ras1 Proto-Oncoprotein; C-Has/Bas P21 Protein; P19 H-RasIDX Protein; EC 3.6.5.2; C-BAS/HAS; C-HA-RAS1; H-RASIDX; C-H-RAS; H-Ras-1; C-H-Ras; Ha-Ras; HAMSV; RASH1; CTLO; KRAS Proto-Oncogene, GTPase; K-Ras4B; KRAS1; KRAS2; V-Ki-Ras2 Kirsten Rat Sarcoma 2 Viral Oncogene Homolog; Kirsten Rat Sarcoma Viral Oncogene Homolog; GTPase KRas; C-Ki-Ras; K-Ras 2; RASK2; V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog; Kirsten Rat Sarcoma Viral Proto-Oncogene; Cellular Transforming Proto-Oncogene; Cellular C-Ki-Ras2 Proto-Oncogene; Transforming Protein P21; PR310 C-K-Ras Oncogene; C-Kirsten-Ras Protein; Proto-Oncogene GTPase; K-Ras P21 Protein; Oncogene KRAS2; EC 3.6.5.2; C-Ki-Ras2; 'C-K-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; C-K-Ras; KI-RAS; Ki-Ras; K-Ras; CFC2; RALD; NS3; OES; NS
Background

Gene Name: NRAS/HRAS/KRAS

NCBI Gene Entry: 4893 , 3265 , 3845

UniProt Entry: P01111 , P01112 , P01116

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB220

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human KRAS+HRAS+NRAS
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using KRAS+HRAS+NRAS antibody (Cat#2303). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRAS+HRAS+NRAS antibody (Cat#2303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells with KRAS+HRAS+NRAS antibody (Cat#2303, 1:1,000). Nuclei were stained blue with DAPI; KRAS+HRAS+NRAS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of KRAS+HRAS+NRAS expression in HeLa cells using KRAS+HRAS+NRAS antibody (Cat#2303, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, KRAS+HRAS+NRAS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NRAS/HRAS/KRAS
  • Catalog：
    2303
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using KRAS+HRAS+NRAS antibody (Cat#2303). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRAS+HRAS+NRAS antibody (Cat#2303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells with KRAS+HRAS+NRAS antibody (Cat#2303, 1:1,000). Nuclei were stained blue with DAPI; KRAS+HRAS+NRAS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of KRAS+HRAS+NRAS expression in HeLa cells using KRAS+HRAS+NRAS antibody (Cat#2303, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, KRAS+HRAS+NRAS.
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