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Home>Product>Primary Antibody

Phospho-c-Myc (T58) Recombinant Rabbit mAb

#2305

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Recombinant Antibody
Western blotting analysis using Phospho-c-Myc (T58) antibody (Cat#2305). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-c-Myc (T58) antibody (Cat#2305, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Phospho-c-Myc (T58) antibody (Cat#2305, 1:1,000). Nuclei were stained blue with DAPI; Phospho-c-Myc (T58) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-c-Myc (T58) expression in HAP-1 cells using Phospho-c-Myc (T58) antibody (Cat#2305, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-c-Myc (T58).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MYC
  • Catalog：
    2305
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name Phospho-c-Myc (T58) Recombinant Rabbit mAb
Aliases MYC Proto-Oncogene, BHLH Transcription Factor; BHLHe39; C-Myc; MYCC; V-Myc Avian Myelocytomatosis Viral Oncogene Homolog; Class E Basic Helix-Loop-Helix Protein 39; Myc Proto-Oncogene Protein; Transcription Factor P64; Proto-Oncogene C-Myc; Myc-Related Translation/Localization Regulatory Factor; Avian Myelocytomatosis Viral Oncogene Homolog; V-Myc Myelocytomatosis Viral Oncogene Homolog; BHLHE39; MRTL
Background

Gene Name: MYC

NCBI Gene Entry: 4609

UniProt Entry: P01106

Application Information

Molecular Weight: Predicted, 51 kDa; observed, 57 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB230

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-c-Myc (T58)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-c-Myc (T58) antibody (Cat#2305). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-c-Myc (T58) antibody (Cat#2305, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with Phospho-c-Myc (T58) antibody (Cat#2305, 1:1,000). Nuclei were stained blue with DAPI; Phospho-c-Myc (T58) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-c-Myc (T58) expression in HAP-1 cells using Phospho-c-Myc (T58) antibody (Cat#2305, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-c-Myc (T58).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MYC
  • Catalog：
    2305
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using Phospho-c-Myc (T58) antibody (Cat#2305). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-c-Myc (T58) antibody (Cat#2305, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Phospho-c-Myc (T58) antibody (Cat#2305, 1:1,000). Nuclei were stained blue with DAPI; Phospho-c-Myc (T58) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-c-Myc (T58) expression in HAP-1 cells using Phospho-c-Myc (T58) antibody (Cat#2305, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-c-Myc (T58).
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