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Poly(ADP-Ribose) Polymerase 2 Recombinant Rabbit mAb

#2378

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Recombinant Antibody
Western blotting analysis using PARP2 antibody (Cat#2378). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARP2 antibody (Cat#2378, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PARP2 expression in HT-1080 cells using PARP2 antibody (Cat#2378, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PARP2.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PARP2
  • Catalog:
    2378
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name Poly(ADP-Ribose) Polymerase 2 Recombinant Rabbit mAb
Aliases Poly(ADP-Ribose) Polymerase 2; ARTD2; ADPRTL2; ADP-Ribosyltransferase (NAD+; Poly(ADP-Ribose) Polymerase)-Like 2; ADP-Ribosyltransferase Diphtheria Toxin-Like 2; Poly (ADP-Ribose) Polymerase Family, Member 2; Protein Poly-ADP-Ribosyltransferase PARP2; DNA ADP-Ribosyltransferase PARP2; NAD(+) ADP-Ribosyltransferase 2; Poly [ADP-Ribose] Polymerase 2; Poly[ADP-Ribose] Synthase 2; EC 2.4.2.30; PADPRT-2; ADPRT-2; HPARP-2; ADPRT2; PARP-2; Poly (ADP-Ribosyl) Transferase-Like 2; Poly[ADP-Ribose] Synthetase 2; EC 2.4.2.-; ADPRTL3
Background

Gene Name: PARP2

NCBI Gene Entry: 10038

UniProt Entry: Q9UGN5

Application Information

Molecular Weight: Predicted, 66 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB555

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PARP2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PARP2 antibody (Cat#2378). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARP2 antibody (Cat#2378, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PARP2 expression in HT-1080 cells using PARP2 antibody (Cat#2378, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PARP2.
Western blotting analysis using PARP2 antibody (Cat#2378). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARP2 antibody (Cat#2378, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PARP2 expression in HT-1080 cells using PARP2 antibody (Cat#2378, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PARP2.
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