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PDGFRA Recombinant Rabbit mAb

#2505

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Recombinant Antibody
Western blotting analysis using PDGFRA antibody (Cat#2505). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDGFRA antibody (Cat#2505, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PDGFRA expression in HAP-1 cells using PDGFRA antibody (Cat#2505, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PDGFRA.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PDGFRA
  • Catalog:
    2505
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M
Information
Product Name PDGFRA Recombinant Rabbit mAb
Aliases Platelet Derived Growth Factor Receptor Alpha; PDGFR2; CD140a; Platelet-Derived Growth Factor Receptor, Alpha Polypeptide; Alpha-Type Platelet-Derived Growth Factor Receptor; Platelet-Derived Growth Factor Receptor Alpha; Platelet-Derived Growth Factor Receptor 2; CD140 Antigen-Like Family Member A; CD140a Antigen; PDGF-R-Alpha; EC 2.7.10.1; PDGFR-2; GAS9; Alpha Platelet-Derived Growth Factor Receptor; Platelet-Derived Growth Factor Alpha Receptor; PDGFR-Alpha; RHEPDGFRA; EC 2.7.10
Background

Gene Name: PDGFRA

NCBI Gene Entry: 5156

UniProt Entry: P16234

Application Information

Molecular Weight: Predicted, 123 kDa; observed, 190 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1165

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PDGFR alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PDGFRA antibody (Cat#2505). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDGFRA antibody (Cat#2505, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of PDGFRA expression in HAP-1 cells using PDGFRA antibody (Cat#2505, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PDGFRA.
Western blotting analysis using PDGFRA antibody (Cat#2505). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDGFRA antibody (Cat#2505, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PDGFRA expression in HAP-1 cells using PDGFRA antibody (Cat#2505, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PDGFRA.
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