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Home>Product>Primary Antibody

Neurofilament Medium Chain Recombinant Rabbit mAb

#2530

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Recombinant Antibody
Western blotting analysis using Neurofilament medium chain antibody (Cat#2530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Neurofilament medium chain antibody (Cat#2530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with Neurofilament medium chain antibody (Cat#2530, 1:1,000). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Neurofilament medium chain expression in Hela cells using Neurofilament medium chain antibody (Cat#2530, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Neurofilament medium chain.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEFM
  • Catalog：
    2530
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name Neurofilament Medium Chain Recombinant Rabbit mAb
Aliases NEFM; Neurofilament Medium Chain; NF-M; NFM; NEF3; Neurofilament, Medium Polypeptide 150kDa; Neurofilament Medium Polypeptide; Neurofilament Triplet M Protein; 160 KDa Neurofilament Protein; Neurofilament 3; Neurofilament, Medium Polypeptide; Neurofilament-3 (150 KD Medium); Neurofilament Medium
Background

Gene Name: NEFM

NCBI Gene Entry: 4741

UniProt Entry: P07197

Application Information

Molecular Weight: Predicted, 103 kDa; observed, 160 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1275

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human NEFM
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Neurofilament medium chain antibody (Cat#2530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Neurofilament medium chain antibody (Cat#2530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of Hela cells with Neurofilament medium chain antibody (Cat#2530, 1:1,000). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Neurofilament medium chain expression in Hela cells using Neurofilament medium chain antibody (Cat#2530, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Neurofilament medium chain.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEFM
  • Catalog：
    2530
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using Neurofilament medium chain antibody (Cat#2530). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Neurofilament medium chain antibody (Cat#2530, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with Neurofilament medium chain antibody (Cat#2530, 1:1,000). Nuclei were stained blue with DAPI; Neurofilament medium chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Neurofilament medium chain expression in Hela cells using Neurofilament medium chain antibody (Cat#2530, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Neurofilament medium chain.
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