Western blotting analysis using Regulator of calcineurin 1 antibody (Cat#2543). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Regulator of calcineurin 1 antibody (Cat#2543, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Regulator of calcineurin 1 antibody (Cat#2543, 1:1,000). Nuclei were stained blue with DAPI; Regulator of calcineurin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Regulator of calcineurin 1 expression in C2C12 cells using Regulator of calcineurin 1 antibody (Cat#2543, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Regulator of calcineurin 1.
RCAN1; Regulator Of Calcineurin 1; DSCR1; Myocyte-Enriched Calcineurin-Interacting Protein 1; Down Syndrome Critical Region Gene 1; Calcipressin-1; ADAPT78; MCIP1; CSP1; DSC1; Modulatory Calcineurin-Interacting Protein 1; Down Syndrome Critical Region Protein 1; Calcium And Oxidant-Inducible MRNA; Down Syndrome Candidate Region 1; Near DSCR Proline-Rich Protein; Adapt78; RCN1
Western blotting analysis using Regulator of calcineurin 1 antibody (Cat#2543). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Regulator of calcineurin 1 antibody (Cat#2543, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Regulator of calcineurin 1 antibody (Cat#2543, 1:1,000). Nuclei were stained blue with DAPI; Regulator of calcineurin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Regulator of calcineurin 1 expression in C2C12 cells using Regulator of calcineurin 1 antibody (Cat#2543, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Regulator of calcineurin 1.
Western blotting analysis using Regulator of calcineurin 1 antibody (Cat#2543). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Regulator of calcineurin 1 antibody (Cat#2543, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Regulator of calcineurin 1 antibody (Cat#2543, 1:1,000). Nuclei were stained blue with DAPI; Regulator of calcineurin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Regulator of calcineurin 1 expression in C2C12 cells using Regulator of calcineurin 1 antibody (Cat#2543, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Regulator of calcineurin 1.
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