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PPP1CA + 1CB Recombinant Rabbit mAb

#2555

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Recombinant Antibody
Western blotting analysis using PPP1CA + 1CB antibody (Cat#2555). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1CA + 1CB antibody (Cat#2555, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with PPP1CA + 1CB antibody (Cat#2555, 1:1,000). Nuclei were stained blue with DAPI; PPP1CA + 1CB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PPP1CA + 1CB expression in HepG2 cells using PPP1CA + 1CB antibody (Cat#2555, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPP1CA + 1CB.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    PPP1CA/ PPP1CB
  • Catalog:
    2555
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name PPP1CA + 1CB Recombinant Rabbit mAb
Aliases PPP1CA; Protein Phosphatase 1 Catalytic Subunit Alpha; PP-1A; PP1alpha; PPP1A; PP1A; Serine/Threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit;
Protein Phosphatase 1, Catalytic Subunit, Alpha Isozyme; EC 3.1.3.16; Serine/Threonine Protein Phosphatase PP1-Alpha 1 Catalytic Subunit; Protein Phosphatase 1, Catalytic Subunit, Alpha Isoform; Testicular Tissue Protein Li 155
Background

Gene Name: PPP1CA/ PPP1CB

NCBI Gene Entry: 5499 , 5500

UniProt Entry: P62136 , P62140

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1385

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human PPP1CA + 1CB
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PPP1CA + 1CB antibody (Cat#2555). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1CA + 1CB antibody (Cat#2555, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with PPP1CA + 1CB antibody (Cat#2555, 1:1,000). Nuclei were stained blue with DAPI; PPP1CA + 1CB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PPP1CA + 1CB expression in HepG2 cells using PPP1CA + 1CB antibody (Cat#2555, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPP1CA + 1CB.
Western blotting analysis using PPP1CA + 1CB antibody (Cat#2555). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP1CA + 1CB antibody (Cat#2555, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with PPP1CA + 1CB antibody (Cat#2555, 1:1,000). Nuclei were stained blue with DAPI; PPP1CA + 1CB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PPP1CA + 1CB expression in HepG2 cells using PPP1CA + 1CB antibody (Cat#2555, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PPP1CA + 1CB.
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