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Home>Product>Primary Antibody

SET Nuclear Proto-Oncogene Recombinant Rabbit mAb

#2588

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Recombinant Antibody
Western blotting analysis using SET antibody (Cat#2588). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SET antibody (Cat#2588, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with SET antibody (Cat#2588, 1:1,000). Nuclei were stained blue with DAPI; SET was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SET expression in Hela cells using SET antibody (Cat#2588, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SET.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SET
  • Catalog：
    2588
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name SET Nuclear Proto-Oncogene Recombinant Rabbit mAb
Aliases SET Nuclear Proto-Oncogene; PHAPII; IGAAD; TAF-I; Inhibitor Of Granzyme A-Activated Dnase; HLA-DR-Associated Protein II; TAF-IBETA; IPP2A2; 2PP2A; SET Translocation (Myeloid Leukemia-Associated); Protein Phosphatase Type 2A Inhibitor; Phosphatase 2A Inhibitor I2PP2A; Chromatin Remodelling Factor; Protein SET; Template-Activating Factor-I, Chromatin Remodelling Factor; Inhibitor-2 Of Protein Phosphatase-2A; Template-Activating Factor-I; Template-Activating Factor I; SET Nuclear Oncogene; I-2PP2A; I2PP2A; MRD58
Background

Gene Name: SET

NCBI Gene Entry: 6418

UniProt Entry: Q01105

Application Information

Molecular Weight: Predicted, 33 kDa; observed, 39 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1530

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human SET
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SET antibody (Cat#2588). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SET antibody (Cat#2588, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with SET antibody (Cat#2588, 1:1,000). Nuclei were stained blue with DAPI; SET was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SET expression in Hela cells using SET antibody (Cat#2588, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SET.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SET
  • Catalog：
    2588
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using SET antibody (Cat#2588). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SET antibody (Cat#2588, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with SET antibody (Cat#2588, 1:1,000). Nuclei were stained blue with DAPI; SET was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SET expression in Hela cells using SET antibody (Cat#2588, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SET.
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