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SYNCRIP Recombinant Rabbit mAb

#2669

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Recombinant Antibody
Western blotting analysis using SYNCRIP antibody (Cat#2669). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SYNCRIP antibody (Cat#2669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with SYNCRIP antibody (Cat#2669, 1:1,000). Nuclei were stained blue with DAPI; SYNCRIP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SYNCRIP expression in HepG2 cells using SYNCRIP antibody (Cat#2669, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SYNCRIP.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    SYNCRIP
  • Catalog:
    2669
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name SYNCRIP Recombinant Rabbit mAb
Aliases SYNCRIP; Synaptotagmin Binding Cytoplasmic RNA Interacting Protein; GRY-RBP; NSAP1; Heterogeneous Nuclear Ribonucleoprotein Q; HnRNP-Q; HNRPQ1; HNRNPQ; Glycine- And Tyrosine-Rich RNA-Binding Protein; NS1-Associated Protein 1; DJ3J17.2; Synaptotagmin-Binding, Cytoplasmic RNA-Interacting Protein; HnRNP Q; GRYRBP; HNRPQ; PP68
Background

Gene Name: SYNCRIP

NCBI Gene Entry: 10492

UniProt Entry: O60506

Application Information

Molecular Weight: Predicted, 70 kDa; observed, 65-82 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2445

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human hnRNP Q
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SYNCRIP antibody (Cat#2669). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SYNCRIP antibody (Cat#2669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with SYNCRIP antibody (Cat#2669, 1:1,000). Nuclei were stained blue with DAPI; SYNCRIP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SYNCRIP expression in HepG2 cells using SYNCRIP antibody (Cat#2669, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SYNCRIP.
Western blotting analysis using SYNCRIP antibody (Cat#2669). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SYNCRIP antibody (Cat#2669, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with SYNCRIP antibody (Cat#2669, 1:1,000). Nuclei were stained blue with DAPI; SYNCRIP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SYNCRIP expression in HepG2 cells using SYNCRIP antibody (Cat#2669, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SYNCRIP.
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