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Home>Product>Primary Antibody

Phospho-SMC1 (S957) Recombinant Rabbit mAb

#2703

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Recombinant Antibody
Western blotting analysis using Phospho-SMC1 (S957) antibody (Cat#2703). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-SMC1 (S957) antibody (Cat#2703, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Phospho-SMC1 (S957) antibody (Cat#2703, 1:1,000). Nuclei were stained blue with DAPI; Phospho-SMC1 (S957) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-SMC1 (S957) expression in HAP-1 cells using Phospho-SMC1 (S957) antibody (Cat#2703, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-SMC1 (S957).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMC1A
  • Catalog：
    2703
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Phospho-SMC1 (S957) Recombinant Rabbit mAb
Aliases SMC1A; Structural Maintenance Of Chromosomes 1A; DXS423E; SB1.8; KIAA0178; SMC1L1; Smcb; SMC1 (Structural Maintenance Of Chromosomes 1, Yeast)-Like 1; Structural Maintenance Of Chromosomes Protein 1A; SMC Protein 1A; SMC-1-Alpha; SMC1; SMC1 Structural Maintenance Of Chromosomes 1-Like 1 (Yeast); Epididymis Secretory Sperm Binding Protein; Segregation Of Mitotic Chromosomes 1; SMC1alpha; EIEE85; SMC-1A; CDLS2; DEE85; Sb1.8
Background

Gene Name: SMC1A

NCBI Gene Entry: 8243

UniProt Entry: Q14683

Application Information

Molecular Weight: Predicted, 143 kDa; observed, 145 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2050

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-SMC1 (S957)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-SMC1 (S957) antibody (Cat#2703). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-SMC1 (S957) antibody (Cat#2703, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with Phospho-SMC1 (S957) antibody (Cat#2703, 1:1,000). Nuclei were stained blue with DAPI; Phospho-SMC1 (S957) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-SMC1 (S957) expression in HAP-1 cells using Phospho-SMC1 (S957) antibody (Cat#2703, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-SMC1 (S957).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMC1A
  • Catalog：
    2703
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using Phospho-SMC1 (S957) antibody (Cat#2703). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-SMC1 (S957) antibody (Cat#2703, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Phospho-SMC1 (S957) antibody (Cat#2703, 1:1,000). Nuclei were stained blue with DAPI; Phospho-SMC1 (S957) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-SMC1 (S957) expression in HAP-1 cells using Phospho-SMC1 (S957) antibody (Cat#2703, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-SMC1 (S957).
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