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ZBTB7A Recombinant Rabbit mAb

#2726

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Recombinant Antibody
Western blotting analysis using ZBTB7A antibody (Cat#2726). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZBTB7A antibody (Cat#2726, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ZBTB7A antibody (Cat#2726, 1:1,000). Nuclei were stained blue with DAPI; ZBTB7A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ZBTB7A expression in HepG2 cells using ZBTB7A antibody (Cat#2726, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ZBTB7A.
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Unit price:
$69.00
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1
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  • Gene Symbol:
    ZBTB7A
  • Catalog:
    2726
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name ZBTB7A Recombinant Rabbit mAb
Aliases ZBTB7A; Zinc Finger And BTB Domain Containing 7A; Pokemon; ZNF857A; FBI-1; LRF; POK Erythroid Myeloid Ontogenic Factor; ZBTB7; Zinc Finger And BTB Domain Containing 7A, HIV-1 Inducer Of Short Transcripts Binding Protein; Factor That Binds To Inducer Of Short Transcripts Protein 1; POZ And Krueppel Erythroid Myeloid Ontogenic Factor; Zinc Finger And BTB Domain-Containing Protein 7A; Leukemia/Lymphoma-Related Factor; TTF-I-Interacting Peptide 21; Factor Binding IST Protein 1; HIV-1 1st-Binding Protein 1; Zinc Finger Protein 857A; Lymphoma Related Factor; DKFZp547O146; Pokemon 1; TIP21; FBI1; HIV-1 Inducer Of Short Transcripts Binding Protein; Zinc Finger And BTB Domain Containing 7; Leukemia/Lymphoma Related Factor; MNDLFH
Background

Gene Name: ZBTB7A

NCBI Gene Entry: 51341

UniProt Entry: O95365

Application Information

Molecular Weight: Predicted, 61 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2395

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human ZBTB7A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ZBTB7A antibody (Cat#2726). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZBTB7A antibody (Cat#2726, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with ZBTB7A antibody (Cat#2726, 1:1,000). Nuclei were stained blue with DAPI; ZBTB7A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ZBTB7A expression in HepG2 cells using ZBTB7A antibody (Cat#2726, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ZBTB7A.
Western blotting analysis using ZBTB7A antibody (Cat#2726). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZBTB7A antibody (Cat#2726, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ZBTB7A antibody (Cat#2726, 1:1,000). Nuclei were stained blue with DAPI; ZBTB7A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ZBTB7A expression in HepG2 cells using ZBTB7A antibody (Cat#2726, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ZBTB7A.
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