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Home>Product>Primary Antibody

Ubiquitin Like Modifier Activating Enzyme 1 Recombinant Rabbit mAb

#2729

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Recombinant Antibody
Western blotting analysis using Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:1.000). Nuclei were stained blue with DAPI; Ubiquitin like modifier activating enzyme 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Ubiquitin like modifier activating enzyme 1 expression in HepG2 cells using Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Ubiquitin like modifier activating enzyme 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBA1
  • Catalog：
    2729
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Ubiquitin Like Modifier Activating Enzyme 1 Recombinant Rabbit mAb
Aliases UBA1; Ubiquitin Like Modifier Activating Enzyme 1; CFAP124; UBE1X; POC20; A1S9T; UBE1; Ubiquitin-Like Modifier-Activating Enzyme 1; Ubiquitin-Activating Enzyme E1; GXP1; Ubiquitin-Activating Enzyme E1 (A1S9T And BN75 Temperature Sensitivity Complementing); UBA1, Ubiquitin-Activating Enzyme E1 Homolog (Yeast); A1S9T And BN75 Temperature Sensitivity Complementing; POC20 Centriolar Protein Homolog (Chlamydomonas); UBA1, Ubiquitin-Activating Enzyme E1 Homolog A; Ubiquitin-Like Modifier Activating Enzyme 1; Testicular Secretory Protein Li 63; POC20 Centriolar Protein Homolog; Protein A1S9; EC 6.2.1.45; AMCX1; SMAX2; UBA1A; VEXAS; A1S9; A1ST
Background

Gene Name: UBA1

NCBI Gene Entry: 7317

UniProt Entry: P22314

Application Information

Molecular Weight: Predicted, 118 kDa; observed, 118 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2435

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human E1 Ubiquitin Activating Enzyme
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:1.000). Nuclei were stained blue with DAPI; Ubiquitin like modifier activating enzyme 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Ubiquitin like modifier activating enzyme 1 expression in HepG2 cells using Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Ubiquitin like modifier activating enzyme 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UBA1
  • Catalog：
    2729
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:1.000). Nuclei were stained blue with DAPI; Ubiquitin like modifier activating enzyme 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Ubiquitin like modifier activating enzyme 1 expression in HepG2 cells using Ubiquitin like modifier activating enzyme 1 antibody (Cat#2729, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Ubiquitin like modifier activating enzyme 1.
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