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TRPM8 Recombinant Rabbit mAb

#2776

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Recombinant Antibody
Western blotting analysis using TRPM8 antibody (Cat#2776). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRPM8 antibody (Cat#2776, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TRPM8 expression in HepG2 cells using TRPM8 antibody (Cat#2776, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TRPM8.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    TRPM8
  • Catalog:
    2776
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name TRPM8 Recombinant Rabbit mAb
Aliases TRPM8; Transient Receptor Potential Cation Channel Subfamily M Member 8; Transient Receptor Potential P8; LTrpC-6; LTRPC6; Trp-P8; TRPP8; Transient Receptor Potential Cation Channel, Subfamily M, Member 8; Transient Receptor Potential Subfamily M Member 8; Long Transient Receptor Potential Channel 6; TRPM8 Cationic Channel; LTrpC6
Background

Gene Name: TRPM8

NCBI Gene Entry: 79054

UniProt Entry: Q7Z2W7

Application Information

Molecular Weight: Predicted, 128 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2225

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human TRPM8
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using TRPM8 antibody (Cat#2776). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRPM8 antibody (Cat#2776, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of TRPM8 expression in HepG2 cells using TRPM8 antibody (Cat#2776, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TRPM8.
Western blotting analysis using TRPM8 antibody (Cat#2776). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRPM8 antibody (Cat#2776, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TRPM8 expression in HepG2 cells using TRPM8 antibody (Cat#2776, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TRPM8.
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