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TAB3 Recombinant Rabbit mAb

#2799

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Recombinant Antibody
Western blotting analysis using TAB3 antibody (Cat#2799). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TAB3 antibody (Cat#2799, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with TAB3 antibody (Cat#2799, 1:1,000). Nuclei were stained blue with DAPI; TAB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TAB3 expression in HT-1080 cells using TAB3 antibody (Cat#2799, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TAB3.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    TAB3
  • Catalog:
    2799
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M
Information
Product Name TAB3 Recombinant Rabbit mAb
Aliases TAB3; TGF-Beta Activated Kinase 1 (MAP3K7) Binding Protein 3; MAP3K7IP3; Mitogen-Activated Protein Kinase Kinase Kinase 7 Interacting Protein 3; TGF-Beta Activated Kinase 1 And MAP3K7 Binding Protein 3; TGF-Beta-Activated Kinase 1 And MAP3K7-Binding Protein 3; TGF-Beta-Activated Kinase 1-Binding Protein 3; NF-Kappa-B-Activating Protein 1; TAK1-Binding Protein 3; TAB-3; Mitogen-Activated Protein Kinase Kinase Kinase 7-Interacting Protein; TGF-Beta Activated Kinase 1/MAP3K7 Binding Protein 3; NFkB Activating Protein 1; TAK1 Binding Protein 3; NAP1
Background

Gene Name: TAB3

NCBI Gene Entry: 257397

UniProt Entry: Q8N5C8

Application Information

Molecular Weight: Predicted, 79 kDa; observed, 90 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2680

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human TAB3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TAB3 antibody (Cat#2799). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TAB3 antibody (Cat#2799, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with TAB3 antibody (Cat#2799, 1:1,000). Nuclei were stained blue with DAPI; TAB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of TAB3 expression in HT-1080 cells using TAB3 antibody (Cat#2799, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TAB3.
Western blotting analysis using TAB3 antibody (Cat#2799). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TAB3 antibody (Cat#2799, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with TAB3 antibody (Cat#2799, 1:1,000). Nuclei were stained blue with DAPI; TAB3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TAB3 expression in HT-1080 cells using TAB3 antibody (Cat#2799, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TAB3.
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