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Home>Product>Primary Antibody

Membrane Metalloendopeptidase Recombinant Rabbit mAb

#2854

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Recombinant Antibody
Western blotting analysis using CD10 antibody (Cat#2854). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD10 antibody (Cat#2854, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with CD10 antibody (Cat#2854, 1:1,000). Nuclei were stained blue with DAPI; CD10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD10 expression in HT-1080 cells using CD10 antibody (Cat#2854, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD10.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MME
  • Catalog：
    2854
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Membrane Metalloendopeptidase Recombinant Rabbit mAb
Aliases MME; Membrane Metalloendopeptidase; CALLA; NEP; Neprilysin; CD10; Common Acute Lymphocytic Leukemia Antigen; Neutral Endopeptidase 24.11; Skin Fibroblast Elastase; Neutral Endopeptidase; Atriopeptidase; Enkephalinase; EC 3.4.24.11; SFE; Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10); Neprilysin-390; Neprilysin-411; CD10 Antigen; EC 3.4.24; CMT2T; SCA43; EPN
Background

Gene Name: MME

NCBI Gene Entry: 4311

UniProt Entry: P08473

Application Information

Molecular Weight: Predicted, 86 kDa; observed, 85 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2850

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human CD10
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CD10 antibody (Cat#2854). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD10 antibody (Cat#2854, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with CD10 antibody (Cat#2854, 1:1,000). Nuclei were stained blue with DAPI; CD10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CD10 expression in HT-1080 cells using CD10 antibody (Cat#2854, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD10.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MME
  • Catalog：
    2854
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using CD10 antibody (Cat#2854). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD10 antibody (Cat#2854, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with CD10 antibody (Cat#2854, 1:1,000). Nuclei were stained blue with DAPI; CD10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CD10 expression in HT-1080 cells using CD10 antibody (Cat#2854, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CD10.
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