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Home>Product>Primary Antibody

Nuclear Factor Kappa B Subunit 2 Recombinant Rabbit mAb

#2988

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Recombinant Antibody
Western blotting analysis using Nuclear factor kappa B subunit 2 antibody (Cat#2988). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nuclear factor kappa B subunit 2 antibody (Cat#2988, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Nuclear factor kappa B subunit 2 antibody (Cat#2988, 1:1,000). Nuclei were stained blue with DAPI; Nuclear factor kappa B subunit 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Nuclear factor kappa B subunit 2 expression in HepG2 cells using Nuclear factor kappa B subunit 2 antibody (Cat#2988, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Nuclear factor kappa B subunit 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NFKB2
  • Catalog：
    2988
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Nuclear Factor Kappa B Subunit 2 Recombinant Rabbit mAb
Aliases NFKB2; Nuclear Factor Kappa B Subunit 2; P49/P100; LYT-10; NF-KB2; P52; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2 (P49/P100); Lymphocyte Translocation Chromosome 10 Protein; Nuclear Factor NF-Kappa-B P100 Subunit; DNA-Binding Factor KBF2; Oncogene Lyt-10 3 4; LYT10; P105; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells; Nuclear Factor NF-Kappa-B P52 Subunit; Nuclear Factor Kappa-B, Subunit 2; Transcription Factor NFKB2; NFKB, P52/P100 Subunit; CVID10; Lyt10; P100
Background

Gene Name: NFKB2

NCBI Gene Entry: 4791

UniProt Entry: Q00653

Application Information

Molecular Weight: Predicted, 97 kDa; observed, 120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3505

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human NFkB p100 / p52
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Nuclear factor kappa B subunit 2 antibody (Cat#2988). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nuclear factor kappa B subunit 2 antibody (Cat#2988, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Nuclear factor kappa B subunit 2 antibody (Cat#2988, 1:1,000). Nuclei were stained blue with DAPI; Nuclear factor kappa B subunit 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Nuclear factor kappa B subunit 2 expression in HepG2 cells using Nuclear factor kappa B subunit 2 antibody (Cat#2988, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Nuclear factor kappa B subunit 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NFKB2
  • Catalog：
    2988
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Nuclear factor kappa B subunit 2 antibody (Cat#2988). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nuclear factor kappa B subunit 2 antibody (Cat#2988, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Nuclear factor kappa B subunit 2 antibody (Cat#2988, 1:1,000). Nuclei were stained blue with DAPI; Nuclear factor kappa B subunit 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Nuclear factor kappa B subunit 2 expression in HepG2 cells using Nuclear factor kappa B subunit 2 antibody (Cat#2988, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Nuclear factor kappa B subunit 2.
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