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Home>Product>Primary Antibody

SYNE1 Recombinant Rabbit mAb

#3008

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Recombinant Antibody
Western blotting analysis using SYNE1 antibody (Cat#3008). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SYNE1 antibody (Cat#3008, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with SYNE1 antibody (Cat#3008, 1:1,000). Nuclei were stained blue with DAPI; SYNE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SYNE1 expression in HepG2 cells using SYNE1 antibody (Cat#3008, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SYNE1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SYNE1
  • Catalog：
    3008
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name SYNE1 Recombinant Rabbit mAb
Aliases SYNE1; Spectrin Repeat Containing Nuclear Envelope Protein 1; Nesprin-1; Enaptin; MYNE1; Myocyte Nuclear Envelope Protein 1; KIAA0796; DJ45H2.2; C6orf98; SCAR8; ARCA1; Nesp1; CPG2; 8B; Spectrin Repeat Containing, Nuclear Envelope 1; Synaptic Nuclear Envelope Protein 1; KASH Domain-Containing Protein 1; SYNE-1B; KASH1; Nuclear Envelope Spectrin Repeat Protein 1; Nuclear Envelope Spectrin Repeat-1; Chromosome 6 Open Reading Frame 98; Synaptic Nuclei Expressed Gene 1; CPG2 Full Length; EC 4.2.1.49; EC 4.3.1.3; Nesprin 1; KIAA1262; KIAA1756; Myne-1; Syne-1; EDMD4; AMC3; AMCM
Background

Gene Name: SYNE1

NCBI Gene Entry: 23345

UniProt Entry: Q8NF91

Application Information

Molecular Weight: Predicted, 112 kDa; observed, 140 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3605

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Nesprin 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SYNE1 antibody (Cat#3008). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SYNE1 antibody (Cat#3008, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with SYNE1 antibody (Cat#3008, 1:1,000). Nuclei were stained blue with DAPI; SYNE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SYNE1 expression in HepG2 cells using SYNE1 antibody (Cat#3008, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SYNE1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SYNE1
  • Catalog：
    3008
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SYNE1 antibody (Cat#3008). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SYNE1 antibody (Cat#3008, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with SYNE1 antibody (Cat#3008, 1:1,000). Nuclei were stained blue with DAPI; SYNE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SYNE1 expression in HepG2 cells using SYNE1 antibody (Cat#3008, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SYNE1.
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