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Home>Product>Primary Antibody

Phosphodiesterase 4D Recombinant Rabbit mAb

#3106

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Recombinant Antibody
Western blotting analysis using phosphodiesterase 4D antibody (Cat#3106). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphodiesterase 4D antibody (Cat#3106, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with phosphodiesterase 4D antibody (Cat#3106, 1:1,000). Nuclei were stained blue with DAPI; Phosphodiesterase 4D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phosphodiesterase 4D expression in HepG2 cells using phosphodiesterase 4D antibody (Cat#3106, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phosphodiesterase 4D.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PDE4D
  • Catalog：
    3106
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Phosphodiesterase 4D Recombinant Rabbit mAb
Aliases PDE4D; Phosphodiesterase 4D; DPDE3; CAMP-Specific 3',5'-Cyclic Phosphodiesterase 4D; 3',5'-Cyclic-AMP Phosphodiesterase 4D; PDE43; Phosphodiesterase 4D, CAMP-Specific (Dunce (Drosophila)-Homolog Phosphodiesterase E3);
Phosphodiesterase 4D, CAMP-Specific (Phosphodiesterase E3 Dunce Homolog, Drosophila); Phosphodiesterase E3 Dunce Homolog (Drosophila); CAMP-Specific Phosphodiesterase PDE4D6;
Phosphodiesterase 4D, CAMP-Specific; CAMP-Specific Phosphodiesterase 4D; Testicular Tissue Protein Li 136; EC 3.1.4.53; EC 3.1.4.17; EC 3.1.4; ACRDYS2; HSPDE4D; PDE4DN2; STRK1
Background

Gene Name: PDE4D

NCBI Gene Entry: 5144

UniProt Entry: Q08499

Application Information

Molecular Weight: Predicted, 91 kDa; observed, 91 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4095

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human PDE4D
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phosphodiesterase 4D antibody (Cat#3106). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphodiesterase 4D antibody (Cat#3106, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with phosphodiesterase 4D antibody (Cat#3106, 1:1,000). Nuclei were stained blue with DAPI; Phosphodiesterase 4D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of phosphodiesterase 4D expression in HepG2 cells using phosphodiesterase 4D antibody (Cat#3106, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phosphodiesterase 4D.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PDE4D
  • Catalog：
    3106
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using phosphodiesterase 4D antibody (Cat#3106). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphodiesterase 4D antibody (Cat#3106, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with phosphodiesterase 4D antibody (Cat#3106, 1:1,000). Nuclei were stained blue with DAPI; Phosphodiesterase 4D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phosphodiesterase 4D expression in HepG2 cells using phosphodiesterase 4D antibody (Cat#3106, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phosphodiesterase 4D.
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