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Home>Product>Primary Antibody

Fms Related Receptor Tyrosine Kinase 1 Recombinant Rabbit mAb

#3277

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Recombinant Antibody
Western blotting analysis using FLT1 antibody (Cat#3277). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLT1 antibody (Cat#3277, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). FLT1, fms related receptor tyrosine kinase 1.
Immunocytochemical staining of HepG2 cells with FLT1 antibody(Cat#3277, 1:1,000). Nuclei were stained blue with DAPI; FLT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FLT1 expression in HepG2 cells using FLT1 antibody (Cat#3277, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FLT1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FLT1
  • Catalog：
    3277
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Fms Related Receptor Tyrosine Kinase 1 Recombinant Rabbit mAb
Aliases FLT1; Fms Related Receptor Tyrosine Kinase 1; VEGFR1; Vascular Endothelial Growth Factor Receptor 1; Vascular Permeability Factor Receptor; FLT; Fms-Related Tyrosine Kinase 1 (Vascular Endothelial Growth Factor/Vascular Permeability Factor Receptor); Tyrosine-Protein Kinase Receptor FLT; Fms Related Tyrosine Kinase 1; Tyrosine-Protein Kinase FRT; Fms-Like Tyrosine Kinase 1; EC 2.7.10.1; VEGFR-1; FLT-1; Fms-Related Tyrosine Kinase 1; EC 2.7.10; FRT
Background

Gene Name: FLT1

NCBI Gene Entry: 2321

UniProt Entry: P17948

Application Information

Molecular Weight: Predicted, 151 kDa; observed, 151 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4840

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human VEGF Receptor 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using FLT1 antibody (Cat#3277). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLT1 antibody (Cat#3277, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). FLT1, fms related receptor tyrosine kinase 1.
  • Immunocytochemical staining of HepG2 cells with FLT1 antibody(Cat#3277, 1:1,000). Nuclei were stained blue with DAPI; FLT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of FLT1 expression in HepG2 cells using FLT1 antibody (Cat#3277, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FLT1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FLT1
  • Catalog：
    3277
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using FLT1 antibody (Cat#3277). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLT1 antibody (Cat#3277, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). FLT1, fms related receptor tyrosine kinase 1.
Immunocytochemical staining of HepG2 cells with FLT1 antibody(Cat#3277, 1:1,000). Nuclei were stained blue with DAPI; FLT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FLT1 expression in HepG2 cells using FLT1 antibody (Cat#3277, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FLT1.
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