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PPARG Coactivator 1 Alpha Recombinant Rabbit mAb

#3318

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Recombinant Antibody
Western blotting analysis using PGC1 alpha antibody (Cat#3318). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PGC1 alpha antibody (Cat#3318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PGC1 alpha expression in C2C12 cells using PGC1 alpha antibody (Cat#3318, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PGC1 alpha.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PPARGC1A
  • Catalog:
    3318
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name PPARG Coactivator 1 Alpha Recombinant Rabbit mAb
Aliases PPARGC1A; PPARG Coactivator 1 Alpha; PGC1A; PGC1; PGC-1alpha; PPARGC1; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha; PPARgamma Coactivator 1alpha; PPARGC-1-Alpha; PGC-1-Alpha; LEM6; Peroxisome Proliferative Activated Receptor, Gamma, Coactivator 1, Alpha; Peroxisome Proliferator-Activated Receptor Gamma, Coactivator 1 Alpha; Peroxisome Proliferative Activated Receptor, Gamma, Coactivator 1; PPAR Gamma Coactivator Variant Form; PPAR-Gamma Coactivator 1-Alpha; Ligand Effect Modulator-6; Ligand Effect Modulator 6; PPARAGCIalpha; PGC-1(Alpha); L-PGC-1alpha; PPARAGCIα; PPARAGCIA; PGC-1v; PGC1 alpha
Background

Gene Name: PPARGC1A

NCBI Gene Entry: 10891

UniProt Entry: Q9UBK2

Application Information

Molecular Weight: Predicted, 91 kDa; observed, 113 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5035

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human PGC1 alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PGC1 alpha antibody (Cat#3318). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PGC1 alpha antibody (Cat#3318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of PGC1 alpha expression in C2C12 cells using PGC1 alpha antibody (Cat#3318, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PGC1 alpha.
Western blotting analysis using PGC1 alpha antibody (Cat#3318). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PGC1 alpha antibody (Cat#3318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PGC1 alpha expression in C2C12 cells using PGC1 alpha antibody (Cat#3318, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PGC1 alpha.
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