Western blotting analysis using RASEF antibody (Cat#3325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RASEF antibody (Cat#3325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). RASEF, RAS and EF-hand domain containing.
Immunocytochemical staining of HepG2 cells with RASEF antibody(Cat#3325, 1:1,000). Nuclei were stained blue with DAPI; RASEF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RASEF expression in HepG2 cells using RASEF antibody (Cat#3325, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RASEF.
RAS And EF-Hand Domain Containing Recombinant Rabbit mAb
Aliases
RASEF; RAS And EF-Hand Domain Containing; RAB45; TSG; Ras And EF-Hand Domain-Containing Protein; Ras-Related Protein Rab-45; FLJ31614; RAB45, Member RAS Oncogene Family
Western blotting analysis using RASEF antibody (Cat#3325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RASEF antibody (Cat#3325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). RASEF, RAS and EF-hand domain containing.
Immunocytochemical staining of HepG2 cells with RASEF antibody(Cat#3325, 1:1,000). Nuclei were stained blue with DAPI; RASEF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RASEF expression in HepG2 cells using RASEF antibody (Cat#3325, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RASEF.
Western blotting analysis using RASEF antibody (Cat#3325). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RASEF antibody (Cat#3325, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). RASEF, RAS and EF-hand domain containing.
Immunocytochemical staining of HepG2 cells with RASEF antibody(Cat#3325, 1:1,000). Nuclei were stained blue with DAPI; RASEF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RASEF expression in HepG2 cells using RASEF antibody (Cat#3325, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, RASEF.
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