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Home>Product>Primary Antibody

Phospho-MCM2 (S27) Recombinant Rabbit mAb

#3337

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Recombinant Antibody
Western blotting analysis using phospho-MCM2 (S27) antibody (Cat#3337). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MCM2 (S27) antibody (Cat#3337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Phospho-MCM2 (S27) antibody (Cat#3337, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MCM2 (S27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospho-MCM2 (S27) expression in HepG2 cells using phospho-MCM2 (S27) antibody (Cat#3337, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-MCM2 (S27).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MCM2
  • Catalog：
    3337
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-MCM2 (S27) Recombinant Rabbit mAb
Aliases MCM2; Minichromosome Maintenance Complex Component 2; BM28; KIAA0030; D3S3194; DFNA70; Cdc19; CCNL1; CDCL1; Minichromosome Maintenance Protein 2 Homolog; DNA Replication Licensing Factor MCM2; Nuclear Protein BM28; Mitotin; MCM2 Minichromosome Maintenance Deficient 2, Mitotin (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin); Minichromosome Maintenance Deficient 2 (Mitotin); Cell Devision Cycle-Like 1; Cyclin-Like 1; EC 3.6.4.12
Background

Gene Name: MCM2

NCBI Gene Entry: 4171

UniProt Entry: P49736

Application Information

Molecular Weight: Predicted, 102 kDa; observed, 125 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5180

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-MCM2 (S27)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using phospho-MCM2 (S27) antibody (Cat#3337). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MCM2 (S27) antibody (Cat#3337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with Phospho-MCM2 (S27) antibody (Cat#3337, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MCM2 (S27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of phospho-MCM2 (S27) expression in HepG2 cells using phospho-MCM2 (S27) antibody (Cat#3337, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-MCM2 (S27).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MCM2
  • Catalog：
    3337
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using phospho-MCM2 (S27) antibody (Cat#3337). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MCM2 (S27) antibody (Cat#3337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Phospho-MCM2 (S27) antibody (Cat#3337, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MCM2 (S27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospho-MCM2 (S27) expression in HepG2 cells using phospho-MCM2 (S27) antibody (Cat#3337, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-MCM2 (S27).
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