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Home>Product>Primary Antibody

RAD52 Recombinant Rabbit mAb

#3357

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Recombinant Antibody
Western blotting analysis using RAD52 antibody (Cat#3357). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD52 antibody (Cat#3357, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RAD52 antibody (Cat#3357, 1:1,000). Nuclei were stained blue with DAPI; RAD52 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    RAD52
  • Catalog：
    3357
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name RAD52 Recombinant Rabbit mAb
Aliases RAD52; RAD52 Homolog, DNA Repair Protein; DNA Repair Protein RAD52 Homolog; Rhabdomyosarcoma Antigen MU-RMS-40.23; RAD52 (S. Cerevisiae) Homolog; RAD52 Homolog (S. Cerevisiae); Recombination Protein RAD52
Background

Gene Name: RAD52

NCBI Gene Entry: 5893

UniProt Entry: P43351

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 48 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5280

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human RAD52
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RAD52 antibody (Cat#3357). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD52 antibody (Cat#3357, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with RAD52 antibody (Cat#3357, 1:1,000). Nuclei were stained blue with DAPI; RAD52 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RAD52
  • Catalog：
    3357
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using RAD52 antibody (Cat#3357). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD52 antibody (Cat#3357, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with RAD52 antibody (Cat#3357, 1:1,000). Nuclei were stained blue with DAPI; RAD52 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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