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Home>Product>Primary Antibody

Matrix Metallopeptidase 2 Recombinant Rabbit mAb

#3383

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Recombinant Antibody
Western blotting analysis using matrix metallopeptidase 2 antibody (Cat#3383). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with matrix metallopeptidase 2 antibody (Cat#3383, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with Matrix metallopeptidase 2 antibody (Cat#3383, 1:1,000). Nuclei were stained blue with DAPI; Matrix metallopeptidase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of matrix metallopeptidase 2 expression in H9c2 cells using matrix metallopeptidase 2 antibody (Cat#3383, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, matrix metallopeptidase 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MMP2
  • Catalog：
    3383
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R

Information

Data

Information
Product Name Matrix Metallopeptidase 2 Recombinant Rabbit mAb
Aliases MMP2; Matrix Metallopeptidase 2; TBE-1; CLG4A; 72 KDa Type IV Collagenase; Matrix Metalloproteinase-2; EC 3.4.24.24; MMP-2; CLG4; Matrix Metalloproteinase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metallopeptidase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metalloproteinase-II; Collagenase Type IV-A; Neutrophil Gelatinase; 72 KDa Gelatinase; Gelatinase A; EC 3.4.24; MMP-II; MONA
Background

Gene Name: MMP2

NCBI Gene Entry: 4313

UniProt Entry: P08253

Application Information

Molecular Weight: Predicted, 74 kDa; observed, 64 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB5415

Species Reactivity: Mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human MMP2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB):1:2,500-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using matrix metallopeptidase 2 antibody (Cat#3383). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with matrix metallopeptidase 2 antibody (Cat#3383, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of H9C2 cells with Matrix metallopeptidase 2 antibody (Cat#3383, 1:1,000). Nuclei were stained blue with DAPI; Matrix metallopeptidase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of matrix metallopeptidase 2 expression in H9c2 cells using matrix metallopeptidase 2 antibody (Cat#3383, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, matrix metallopeptidase 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MMP2
  • Catalog：
    3383
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R
RELATED PRODUCTS
Western blotting analysis using matrix metallopeptidase 2 antibody (Cat#3383). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with matrix metallopeptidase 2 antibody (Cat#3383, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with Matrix metallopeptidase 2 antibody (Cat#3383, 1:1,000). Nuclei were stained blue with DAPI; Matrix metallopeptidase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of matrix metallopeptidase 2 expression in H9c2 cells using matrix metallopeptidase 2 antibody (Cat#3383, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, matrix metallopeptidase 2.
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