Western blotting analysis using KEAP1 antibody (Cat#3510). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KEAP1 antibody (Cat#3510, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of KEAP1 expression in HAP-1 cells using KEAP1 antibody (Cat#3510, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, KEAP1.
KEAP1; Kelch Like ECH Associated Protein 1; KLHL19; INrf2; KIAA0132; Kelch-Like ECH-Associated Protein 1; Kelch-Like Family Member 19; Cytosolic Inhibitor Of Nrf2; Kelch-Like Protein 19; MGC10630; MGC20887; MGC1114; MGC4407; MGC9454; KEAP1 Delta C; INRF2
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human KEAP1
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using KEAP1 antibody (Cat#3510). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KEAP1 antibody (Cat#3510, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of KEAP1 expression in HAP-1 cells using KEAP1 antibody (Cat#3510, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, KEAP1.
Western blotting analysis using KEAP1 antibody (Cat#3510). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KEAP1 antibody (Cat#3510, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of KEAP1 expression in HAP-1 cells using KEAP1 antibody (Cat#3510, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, KEAP1.
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