Western blotting analysis using DNAJB1 antibody (Cat#3520). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJB1 antibody (Cat#3520, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with DNAJB1 antibody (Cat#3520, 1:1,000). Nuclei were stained blue with DAPI; DNAJB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DNAJB1 expression in HepG2 cells using DNAJB1 antibody (Cat#3520, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DNAJB1.
DNAJB1; DnaJ Heat Shock Protein Family (Hsp40) Member B1; RSPH16B; Hsp40; HSPF1; Sis1; DnaJ (Hsp40) Homolog, Subfamily B, Member 1; DnaJ Homolog Subfamily B Member 1; Heat Shock 40 KDa Protein 1; DnaJ Protein Homolog 1; Human DnaJ Protein 1; Radial Spoke 16 Homolog B (Chlamydomonas); Radial Spoke 16 Homolog B; Heat Shock Protein 40; DNAJ1; HSP40; HDj-1; Hdj1; HDJ1
Western blotting analysis using DNAJB1 antibody (Cat#3520). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJB1 antibody (Cat#3520, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with DNAJB1 antibody (Cat#3520, 1:1,000). Nuclei were stained blue with DAPI; DNAJB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DNAJB1 expression in HepG2 cells using DNAJB1 antibody (Cat#3520, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DNAJB1.
Western blotting analysis using DNAJB1 antibody (Cat#3520). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJB1 antibody (Cat#3520, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with DNAJB1 antibody (Cat#3520, 1:1,000). Nuclei were stained blue with DAPI; DNAJB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of DNAJB1 expression in HepG2 cells using DNAJB1 antibody (Cat#3520, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DNAJB1.
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