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Mannose Phosphate Isomerase Mouse mAb

#3550

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Western blotting analysis using mannose phosphate isomerase antibody (Cat#3550). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mannose phosphate isomerase antibody (Cat#3550, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Mannose phosphate isomerase expression in HeLa cells using Mannose phosphate isomerase antibody (Cat#3550, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Mannose phosphate isomerase.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    MPI
  • Catalog:
    3550
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name Mannose Phosphate Isomerase Mouse mAb
Aliases MPI; Mannose Phosphate Isomerase; Mannose-6-Phosphate Isomerase; Phosphohexomutase; EC 5.3.1.8; PMI1; PMI; Phosphomannose Isomerase 1; Phosphomannose Isomerase; EC 5.3.1; CDG1B
Background

Gene Name: MPI

NCBI Gene Entry: 4351

UniProt Entry: P34949

Application Information

Molecular Weight: Predicted, 47 kDa; observed, 40 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6470

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human MPI
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using mannose phosphate isomerase antibody (Cat#3550). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mannose phosphate isomerase antibody (Cat#3550, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Mannose phosphate isomerase expression in HeLa cells using Mannose phosphate isomerase antibody (Cat#3550, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Mannose phosphate isomerase.
Western blotting analysis using mannose phosphate isomerase antibody (Cat#3550). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mannose phosphate isomerase antibody (Cat#3550, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Mannose phosphate isomerase expression in HeLa cells using Mannose phosphate isomerase antibody (Cat#3550, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Mannose phosphate isomerase.
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