Western blotting analysis using CAMK2B antibody (Cat#3682). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAMK2B antibody (Cat#3682, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CAMK2B expression in HAP-1 cells using CAMK2B antibody (Cat#3682, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CAMK2B.
CAMK2B; Calcium/Calmodulin Dependent Protein Kinase II Beta; CAMK2; CAM2; CAMKB; Calcium/Calmodulin-Dependent Protein Kinase Type II Subunit Beta; Proline Rich Calmodulin-Dependent Protein Kinase; CaM Kinase II Beta Subunit; CaM-Kinase II Beta Chain; CaMK-II Subunit Beta; EC 2.7.11.17; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Beta; Calcium/Calmodulin-Dependent Protein Kinase Type II Beta Chain; CaM Kinase II Subunit Beta; CaMKIIbeta; EC 2.7.11; CaMKIIβ; CAMKIIB; MRD54
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human CaMKII
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using CAMK2B antibody (Cat#3682). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAMK2B antibody (Cat#3682, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CAMK2B expression in HAP-1 cells using CAMK2B antibody (Cat#3682, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CAMK2B.
Western blotting analysis using CAMK2B antibody (Cat#3682). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAMK2B antibody (Cat#3682, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CAMK2B expression in HAP-1 cells using CAMK2B antibody (Cat#3682, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CAMK2B.
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