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Home>Product>Primary Antibody

FHL1 Recombinant Rabbit mAb

#3698

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Recombinant Antibody
Western blotting analysis using FHL1 antibody (Cat#3698). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FHL1 antibody (Cat#3698, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with FHL1 antibody (Cat#3698, 1:1,000). Nuclei were stained blue with DAPI; FHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FHL1 expression in H9c2 cells using FHL1 antibody (Cat#3698, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FHL1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FHL1
  • Catalog：
    3698
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name FHL1 Recombinant Rabbit mAb
Aliases FHL1; Four And A Half LIM Domains 1; SLIM1; FHL1B; XMPMA; FLH1A; Four And A Half LIM Domains Protein 1; Skeletal Muscle LIM-Protein 1; LIM Protein SLIMMER; BA535K18.1; MGC111107; SLIM-1; KYO-T; FHL-1; SLIM; Four-And-A-Half Lin11, Isl-1 And Mec-3 Domains 1; Four-And-A-Half LIM Domains 1; SLIMMER; RBMX1A; RBMX1B; FCMSU; FHL1A; KYOT
Background

Gene Name: FHL1

NCBI Gene Entry: 2273

UniProt Entry: Q13642

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 32 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6965

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human FHL1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using FHL1 antibody (Cat#3698). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FHL1 antibody (Cat#3698, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of H9C2 cells with FHL1 antibody (Cat#3698, 1:1,000). Nuclei were stained blue with DAPI; FHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of FHL1 expression in H9c2 cells using FHL1 antibody (Cat#3698, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FHL1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    FHL1
  • Catalog：
    3698
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using FHL1 antibody (Cat#3698). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FHL1 antibody (Cat#3698, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9C2 cells with FHL1 antibody (Cat#3698, 1:1,000). Nuclei were stained blue with DAPI; FHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FHL1 expression in H9c2 cells using FHL1 antibody (Cat#3698, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FHL1.
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