Western blotting analysis using STAT1 antibody (Cat#3738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT1 antibody (Cat#3738, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAT1, signal transducer and activator of transcription 1.
Immunocytochemical staining of H9C2 cells with STAT1 antibody (Cat#3738, 1:1,000). Nuclei were stained blue with DAPI; STAT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of STAT1 expression in H9c2 cells using STAT1 antibody (Cat#3738, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, STAT1.
STAT1; Signal Transducer And Activator Of Transcription 1; Transcription Factor ISGF-3 Components P91/P84; STAT91; ISGF-3; Signal Transducer And Activator Of Transcription 1-Alpha/Beta; Signal Transducer And Activator Of Transcription 1, 91kDa; Signal Transducer And Activator Of Transcription 1, 91kD; CANDF7; IMD31A; IMD31B; IMD31C
Western blotting analysis using STAT1 antibody (Cat#3738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT1 antibody (Cat#3738, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAT1, signal transducer and activator of transcription 1.
Immunocytochemical staining of H9C2 cells with STAT1 antibody (Cat#3738, 1:1,000). Nuclei were stained blue with DAPI; STAT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of STAT1 expression in H9c2 cells using STAT1 antibody (Cat#3738, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, STAT1.
Western blotting analysis using STAT1 antibody (Cat#3738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT1 antibody (Cat#3738, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAT1, signal transducer and activator of transcription 1.
Immunocytochemical staining of H9C2 cells with STAT1 antibody (Cat#3738, 1:1,000). Nuclei were stained blue with DAPI; STAT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of STAT1 expression in H9c2 cells using STAT1 antibody (Cat#3738, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, STAT1.
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