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Home>Product>Primary Antibody

Alpha Tubulin 4A Recombinant Rabbit mAb

#3791

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Recombinant Antibody
Western blotting analysis using α-Tubulin antibody (Cat#3791). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with α-Tubulin antibody (Cat#3791, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with α-Tubulin antibody (Cat#3791, 1:1,000). Nuclei were stained blue with DAPI; α-Tubulin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Flow cytometric analysis of α-Tubulin expression in HepG2 cells using α-Tubulin antibody (Cat#3791, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, α-Tubulin.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TUBA4A
  • Catalog：
    3791
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Alpha Tubulin 4A Recombinant Rabbit mAb
Aliases TUBA4A; Tubulin Alpha 4a; H2-ALPHA; TUBA1; Tubulin, Alpha 1 (Testis Specific); Tubulin Alpha-4A Chain; Tubulin Alpha-1 Chain; Tubulin H2-Alpha; FLJ30169; Testis-Specific Alpha-Tubulin; Tubulin, Alpha 1; Alpha-Tubulin 1; EC 3.6.5.-; ALS22
Background

Gene Name: TUBA4A

NCBI Gene Entry: 7277

UniProt Entry: P68366

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB7890

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human alpha 4A Tubulin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using α-Tubulin antibody (Cat#3791). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with α-Tubulin antibody (Cat#3791, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with α-Tubulin antibody (Cat#3791, 1:1,000). Nuclei were stained blue with DAPI; α-Tubulin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
  • Flow cytometric analysis of α-Tubulin expression in HepG2 cells using α-Tubulin antibody (Cat#3791, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, α-Tubulin.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TUBA4A
  • Catalog：
    3791
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using α-Tubulin antibody (Cat#3791). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with α-Tubulin antibody (Cat#3791, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with α-Tubulin antibody (Cat#3791, 1:1,000). Nuclei were stained blue with DAPI; α-Tubulin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol. Scale bar, 20 μm.
Flow cytometric analysis of α-Tubulin expression in HepG2 cells using α-Tubulin antibody (Cat#3791, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, α-Tubulin.
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