Western blotting analysis using EFHD1 antibody (Cat#3885). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EFHD1 antibody (Cat#3885, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EFHD1 expression in HeLa cells using EFHD1 antibody (Cat#3885, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EFHD1.
EFHD1; EF-Hand Domain Family Member D1; Swiprosin-2; EF-Hand Domain-Containing Protein D1; EF-Hand Domain-Containing Protein 1; FLJ13612; SWS2; EF Hand Domain Containing 1; MSTP133; MST133; PP3051
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human EFHD1
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:100-1:500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using EFHD1 antibody (Cat#3885). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EFHD1 antibody (Cat#3885, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EFHD1 expression in HeLa cells using EFHD1 antibody (Cat#3885, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EFHD1.
Western blotting analysis using EFHD1 antibody (Cat#3885). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EFHD1 antibody (Cat#3885, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EFHD1 expression in HeLa cells using EFHD1 antibody (Cat#3885, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, EFHD1.
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