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CC2D1A Recombinant Rabbit mAb

#3929

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Recombinant Antibody
Western blotting analysis using CC2D1A antibody (Cat#3929). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CC2D1A antibody (Cat#3929, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with CC2D1A antibody (Cat#3929, 1:1,000). Nuclei were stained blue with DAPI; CC2D1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CC2D1A expression in C2C12 cells using CC2D1A antibody (Cat#3929, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CC2D1A.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    CC2D1A
  • Catalog:
    3929
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name CC2D1A Recombinant Rabbit mAb
Aliases CC2D1A; Coiled-Coil And C2 Domain Containing 1A; Five Prime Repressor Element Under Dual; Repression-Binding Protein 1; Akt Kinase-Interacting Protein 1; MRT3; TAPE; Coiled-Coil And C2 Domain-Containing Protein 1A; FRE Under Dual Repression-Binding Protein 1; Putative NF-Kappa-B-Activating Protein 023N; TBK1-Associated Protein In Endolysosomes 2; Lethal (2) Giant Discs Homolog 2; FLJ20241; Freud-1; FREUD-1; Aki-1; Lgd2; Five Repressor Element Under Dual Repression-Binding Protein 1; Mental Retardation, Nonsyndromic, Autosomal Recessive, 3; Putative NFkB Activating Protein; Freud-1/Aki1; AKI-1; LGD2; AKI1
Background

Gene Name: CC2D1A

NCBI Gene Entry: 54862

UniProt Entry: Q6P1N0

Application Information

Molecular Weight: Predicted, 104 kDa; observed, 130 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB8735

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from CC2D1A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using CC2D1A antibody (Cat#3929). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CC2D1A antibody (Cat#3929, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with CC2D1A antibody (Cat#3929, 1:1,000). Nuclei were stained blue with DAPI; CC2D1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of CC2D1A expression in C2C12 cells using CC2D1A antibody (Cat#3929, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CC2D1A.
Western blotting analysis using CC2D1A antibody (Cat#3929). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CC2D1A antibody (Cat#3929, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with CC2D1A antibody (Cat#3929, 1:1,000). Nuclei were stained blue with DAPI; CC2D1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of CC2D1A expression in C2C12 cells using CC2D1A antibody (Cat#3929, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CC2D1A.
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