Western blotting analysis using phospholipid scramblase 3 antibody (Cat#3999). Total cell lysates (20 μg for H9c2 and C2C12, 30 μg for others ) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospholipid scramblase 3 antibody (Cat#3999, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with Phospholipid scramblase 3 antibody (Cat#3999, 1:1,000). Nuclei were stained blue with DAPI;Phospholipid scramblase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospholipid scramblase 3 expression in H9c2 cells using phospholipid scramblase 3 antibody (Cat#3999, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, phospholipid scramblase 3.
Western blotting analysis using phospholipid scramblase 3 antibody (Cat#3999). Total cell lysates (20 μg for H9c2 and C2C12, 30 μg for others ) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospholipid scramblase 3 antibody (Cat#3999, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with Phospholipid scramblase 3 antibody (Cat#3999, 1:1,000). Nuclei were stained blue with DAPI;Phospholipid scramblase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospholipid scramblase 3 expression in H9c2 cells using phospholipid scramblase 3 antibody (Cat#3999, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, phospholipid scramblase 3.
Western blotting analysis using phospholipid scramblase 3 antibody (Cat#3999). Total cell lysates (20 μg for H9c2 and C2C12, 30 μg for others ) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospholipid scramblase 3 antibody (Cat#3999, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of H9c2 cells with Phospholipid scramblase 3 antibody (Cat#3999, 1:1,000). Nuclei were stained blue with DAPI;Phospholipid scramblase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of phospholipid scramblase 3 expression in H9c2 cells using phospholipid scramblase 3 antibody (Cat#3999, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, phospholipid scramblase 3.
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