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Home>Product>Primary Antibody

Acyl-CoA Dehydrogenase Short Chain Recombinant Rabbit mAb

#4005

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Recombinant Antibody
Western blotting analysis using acyl-CoA dehydrogenase short chain antibody (Cat#4005). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with acyl-CoA dehydrogenase short chain antibody (Cat#4005, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse liver using acyl-CoA dehydrogenase short chain antibody (Cat#4005, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Acyl-CoA dehydrogenase short chain expression in C2C12 cells using Acyl-CoA dehydrogenase short chain antibody (Cat#4005, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Acyl-CoA dehydrogenase short chain.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ACADS
  • Catalog：
    4005
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Acyl-CoA Dehydrogenase Short Chain Recombinant Rabbit mAb
Aliases ACADS; Acyl-CoA Dehydrogenase Short Chain; SCAD; ACAD3; Short-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; Acyl-Coenzyme A Dehydrogenase, C-2 To C-3 Short Chain; Butyryl-CoA Dehydrogenase; Mitochondrial Short-Chain Specific Acyl-CoA Dehydrogenase; Acyl-CoA Dehydrogenase, C-2 To C-3 Short Chain; Epididymis Secretory Sperm Binding Protein; Unsaturated Acyl-CoA Reductase; EC 1.3.99.2; EC 1.3.8.1; EC 1.3.99
Background

Gene Name: ACADS

NCBI Gene Entry: 35

UniProt Entry: P16219

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9115

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human ACADS
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using acyl-CoA dehydrogenase short chain antibody (Cat#4005). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with acyl-CoA dehydrogenase short chain antibody (Cat#4005, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded mouse liver using acyl-CoA dehydrogenase short chain antibody (Cat#4005, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of Acyl-CoA dehydrogenase short chain expression in C2C12 cells using Acyl-CoA dehydrogenase short chain antibody (Cat#4005, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Acyl-CoA dehydrogenase short chain.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ACADS
  • Catalog：
    4005
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using acyl-CoA dehydrogenase short chain antibody (Cat#4005). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with acyl-CoA dehydrogenase short chain antibody (Cat#4005, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded mouse liver using acyl-CoA dehydrogenase short chain antibody (Cat#4005, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Acyl-CoA dehydrogenase short chain expression in C2C12 cells using Acyl-CoA dehydrogenase short chain antibody (Cat#4005, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Acyl-CoA dehydrogenase short chain.
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