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Home>Product>Primary Antibody

AMPK Alpha 1 Recombinant Rabbit mAb

#4100

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Recombinant Antibody
Western blotting analysis using AMPK alpha 1 antibody (Cat#4100). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AMPK alpha 1 antibody (Cat#4100, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with AMPK alpha 1 antibody (Cat#4100, 1:1,000). Nuclei were stained blue with DAPI; AMPK alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of AMPK alpha 1 expression in HepG2 cells using AMPK alpha 1 antibody (Cat#4100, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AMPK alpha 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRKAA1
  • Catalog：
    4100
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name AMPK Alpha 1 Recombinant Rabbit mAb
Aliases PRKAA1; Protein Kinase AMP-Activated Catalytic Subunit Alpha 1; AMPKa1; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Protein Kinase, AMP-Activated, Alpha 1 Catalytic Subunit; Hydroxymethylglutaryl-CoA Reductase Kinase; Acetyl-CoA Carboxylase Kinase; Tau-Protein Kinase PRKAA1; AMPK Subunit Alpha-1; AMPK, Alpha, 1; ACACA Kinase; HMGCR Kinase; EC 2.7.11.1; 5'-AMP-Activated Protein Kinase, Catalytic Alpha-1 Chain; AMP-Activated Protein Kinase, Catalytic, Alpha-1; AMP -Activate Kinase Alpha 1 Subunit; AMPK Alpha 1; EC 2.7.11.31; EC 2.7.11.26; EC 2.7.11; AMPK1; AMPK
Background

Gene Name: PRKAA1

NCBI Gene Entry: 5562

UniProt Entry: Q13131

Application Information

Molecular Weight: Predicted, 64 kDa; observed, 63 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9590

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human AMPK alpha 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using AMPK alpha 1 antibody (Cat#4100). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AMPK alpha 1 antibody (Cat#4100, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with AMPK alpha 1 antibody (Cat#4100, 1:1,000). Nuclei were stained blue with DAPI; AMPK alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of AMPK alpha 1 expression in HepG2 cells using AMPK alpha 1 antibody (Cat#4100, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AMPK alpha 1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRKAA1
  • Catalog：
    4100
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using AMPK alpha 1 antibody (Cat#4100). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AMPK alpha 1 antibody (Cat#4100, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with AMPK alpha 1 antibody (Cat#4100, 1:1,000). Nuclei were stained blue with DAPI; AMPK alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of AMPK alpha 1 expression in HepG2 cells using AMPK alpha 1 antibody (Cat#4100, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AMPK alpha 1.
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