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Home>Product>Primary Antibody

ABCB1 Recombinant Rabbit mAb

#4182

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Recombinant Antibody
Western blotting analysis using ABCB1 antibody (Cat#4182). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#4182, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse liver using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ABCB1
  • Catalog：
    4182
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ABCB1 Recombinant Rabbit mAb
Aliases ABCB1; ATP Binding Cassette Subfamily B Member 1; PGY1; Multidrug Resistance Protein 1; CD243; GP170; ABC20; P-170; MDR1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; ATP-Dependent Translocase ABCB1; Phospholipid Transporter ABCB1; Colchicin Sensitivity; P-Glycoprotein 1; CLCS; P-GP; ATP-Binding Cassette Sub-Family B Member 1; Doxorubicin Resistance; P-Glycoprotein; CD243 Antigen; EC 3.6.3.44; EC 7.6.2.2; EC 7.6.2.1; EC 3.6.3; P-Gp
Background

Gene Name: ABCB1

NCBI Gene Entry: 5243

UniProt Entry: P08183

Application Information

Molecular Weight: Predicted, 141 kDa; observed, 180 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB9950

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human P Glycoprotein
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using ABCB1 antibody (Cat#4182). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#4182, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded mouse liver using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse kidney using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ABCB1
  • Catalog：
    4182
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ABCB1 antibody (Cat#4182). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#4182, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse liver using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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